Western Blot was performed on nuclear extracts from HeLa cells (HeLa NE, 20 µg) using a SAP30 polyclonal antibody (Product # PA5-40068) at a dilution of 1:1000 in TBS-Tween containing 5% skimmed milk. The molecular weight marker (kDa) is shown on the left; the location of the protein of interest is indicated on the right.